Ryanair, which employs around 1,500 people in Britain, has this year laid out a range of proposals aimed at cutting costs across the business and offering cheaper flights than its rivals.
Included in these plans are designated standing areas on flights across Europe.
Last night in an interview on BBC's Newsnight, he defended the airline’s strategy, saying there are "no hidden costs" on Ryanair and that "everything we do is to lower costs".
Asked whether he would be charging customers £1 to use the toilet on board a flight, he said: "I hope so".
Ryanair believes that charging customers to make use of the facilities will encourage travellers on one-hour flights to use lavatories at the airport instead of on the aircraft.
O’Leary's decision to introduce vertical seats, which would allow passengers to be strapped in while standing up, is a controversial one and could struggle to meet safety requirements.
O'Leary denied that Ryanair is on the acquisition trail, saying there were "not other airlines to buy in Europe".