The ad, which first aired in the UK in 1989, will return to air from 18 November as the retailer celebrates its 25th Christmas in the UK.
The retailer operates 75 stores across the UK and said it has decided to air the ad because of customer feedback.
Mike Coogan, marketing director at Toys R Us said: ‘The Magical Place TV commercial has become synonymous with the success of Toys R Us. We are absolutely thrilled by the overwhelming interest we have received on YouTube and Facebook and are delighted to bring back this iconic commercial.'
The ad has had 200,000 viewings on YouTube and Geoffrey, the Toy R US brand character, has thousands of fans on Facebook.